Construct: shRNA TRCN0000112106
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_025991.1-1193s1c1
- DNA Barcode:
- GCAGTTATCTACTATCGGGTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Kbtbd4 (67136)
- Gene Description:
- kelch repeat and BTB (POZ) domain containing 4
- Transcript:
- RefSeq NM_025991.1 (NON-CURRENT)
- Match location:
- Position 1193 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000112106)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAGTTATCTACTATCGGGTA
- Hairpin Sequence:
-
5'-CCGG-GCAGTTATCTACTATCGGGTA-CTCGAG-TACCCGATAGTAGATAACTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGTTATCTACTATCGGGTACTCGAGTACCCGATAGTAGATAACTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGTTATCTACTATCGGGTACTCGAGTACCCGATAGTAGATAACTGC-3'
Other clones with same target sequence:
-
TRCN0000308919
