Construct: shRNA TRCN0000112107

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025991.1-364s1c1
DNA Barcode:
CCGATCCATGTTCACATCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kbtbd4 (67136)
Gene Description:
kelch repeat and BTB (POZ) domain containing 4
Transcript:
RefSeq NM_025991.1 (NON-CURRENT)
Match location:
Position 364 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112107)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGATCCATGTTCACATCCAA
Hairpin Sequence:
5'-CCGG-CCGATCCATGTTCACATCCAA-CTCGAG-TTGGATGTGAACATGGATCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGATCCATGTTCACATCCAACTCGAGTTGGATGTGAACATGGATCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGATCCATGTTCACATCCAACTCGAGTTGGATGTGAACATGGATCGG-3'

Other clones with same target sequence:

  1. TRCN0000308918