Construct: shRNA TRCN0000112140

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009517.1-7208s1c1
DNA Barcode:
GCCGAGAGAATGTGAACCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zmat3 (22401)
Gene Description:
zinc finger matrin type 3
Transcript:
RefSeq NM_009517.1 (NON-CURRENT)
Match location:
Position 7208 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112140)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAGAGAATGTGAACCTTT
Hairpin Sequence:
5'-CCGG-GCCGAGAGAATGTGAACCTTT-CTCGAG-AAAGGTTCACATTCTCTCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAGAGAATGTGAACCTTTCTCGAGAAAGGTTCACATTCTCTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAGAGAATGTGAACCTTTCTCGAGAAAGGTTCACATTCTCTCGGC-3'

Other clones with same target sequence:

(none)