Construct: shRNA TRCN0000112155

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173071.1-1716s1c1
DNA Barcode:
CCAGCTTTGCTCGTTGCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgrb2 (230775)
Gene Description:
adhesion G protein-coupled receptor B2
Transcript:
RefSeq NM_173071.1 (NON-CURRENT)
Match location:
Position 1716 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112155)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCTTTGCTCGTTGCATAT
Hairpin Sequence:
5'-CCGG-CCAGCTTTGCTCGTTGCATAT-CTCGAG-ATATGCAACGAGCAAAGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCTTTGCTCGTTGCATATCTCGAGATATGCAACGAGCAAAGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCTTTGCTCGTTGCATATCTCGAGATATGCAACGAGCAAAGCTGG-3'

Other clones with same target sequence:

(none)