Construct: shRNA TRCN0000112161

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175642.2-3370s1c1
DNA Barcode:
CGCTCCATATTGTTTCAGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgrb3 (210933)
Gene Description:
adhesion G protein-coupled receptor B3
Transcript:
RefSeq NM_175642.2 (NON-CURRENT)
Match location:
Position 3370 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112161)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTCCATATTGTTTCAGATA
Hairpin Sequence:
5'-CCGG-CGCTCCATATTGTTTCAGATA-CTCGAG-TATCTGAAACAATATGGAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTCCATATTGTTTCAGATACTCGAGTATCTGAAACAATATGGAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTCCATATTGTTTCAGATACTCGAGTATCTGAAACAATATGGAGCG-3'

Other clones with same target sequence:

(none)