Construct: shRNA TRCN0000112212

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011339.1-258s1c1
DNA Barcode:
CCCAATTACTAACAGGTTCCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cxcl15 (20309)
Gene Description:
chemokine (C-X-C motif) ligand 15
Transcript:
RefSeq NM_011339.1 (NON-CURRENT)
Match location:
Position 258 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112212)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATTACTAACAGGTTCCT
Hairpin Sequence:
5'-CCGG-CCCAATTACTAACAGGTTCCT-CTCGAG-AGGAACCTGTTAGTAATTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATTACTAACAGGTTCCTCTCGAGAGGAACCTGTTAGTAATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATTACTAACAGGTTCCTCTCGAGAGGAACCTGTTAGTAATTGGG-3'

Other clones with same target sequence:

  1. TRCN0000314381