Construct: shRNA TRCN0000112254

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153505.2-246s1c1
DNA Barcode:
CCAACATAGATGTCCGAAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nckap1l (105855)
Gene Description:
NCK associated protein 1 like
Transcript:
RefSeq NM_153505.2 (NON-CURRENT)
Match location:
Position 246 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112254)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACATAGATGTCCGAAATA
Hairpin Sequence:
5'-CCGG-CCAACATAGATGTCCGAAATA-CTCGAG-TATTTCGGACATCTATGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACATAGATGTCCGAAATACTCGAGTATTTCGGACATCTATGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACATAGATGTCCGAAATACTCGAGTATTTCGGACATCTATGTTGG-3'

Other clones with same target sequence:

(none)