Construct: shRNA TRCN0000112268

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011351.1-691s1c1
DNA Barcode:
GCCACACTTTGTCTATGCGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sema6c (20360)
Gene Description:
sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C
Transcript:
RefSeq NM_011351.1 (NON-CURRENT)
Match location:
Position 691 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112268)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACACTTTGTCTATGCGTT
Hairpin Sequence:
5'-CCGG-GCCACACTTTGTCTATGCGTT-CTCGAG-AACGCATAGACAAAGTGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACACTTTGTCTATGCGTTCTCGAGAACGCATAGACAAAGTGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACACTTTGTCTATGCGTTCTCGAGAACGCATAGACAAAGTGTGGC-3'

Other clones with same target sequence:

(none)