Construct: shRNA TRCN0000112269

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011351.1-1540s1c1
DNA Barcode:
CTTTCCTGGATGTATCGTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sema6c (20360)
Gene Description:
sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C
Transcript:
RefSeq NM_011351.1 (NON-CURRENT)
Match location:
Position 1540 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112269)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTCCTGGATGTATCGTCTA
Hairpin Sequence:
5'-CCGG-CTTTCCTGGATGTATCGTCTA-CTCGAG-TAGACGATACATCCAGGAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTCCTGGATGTATCGTCTACTCGAGTAGACGATACATCCAGGAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTCCTGGATGTATCGTCTACTCGAGTAGACGATACATCCAGGAAAG-3'

Other clones with same target sequence:

(none)