Construct: shRNA TRCN0000112293

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013659.1-2022s1c1
DNA Barcode:
GCCATGTAAACAAGTCCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sema4b (20352)
Gene Description:
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
Transcript:
RefSeq NM_013659.1 (NON-CURRENT)
Match location:
Position 2022 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112293)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGTAAACAAGTCCAGAT
Hairpin Sequence:
5'-CCGG-GCCATGTAAACAAGTCCAGAT-CTCGAG-ATCTGGACTTGTTTACATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGTAAACAAGTCCAGATCTCGAGATCTGGACTTGTTTACATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGTAAACAAGTCCAGATCTCGAGATCTGGACTTGTTTACATGGC-3'

Other clones with same target sequence:

  1. TRCN0000326923