Construct: shRNA TRCN0000112316

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018744.2-3411s1c1
DNA Barcode:
GCTCGAATATAAGAGGAGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sema6a (20358)
Gene Description:
sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
Transcript:
RefSeq NM_018744.2 (CURRENT)
Match location:
Position 3411 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112316)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGAATATAAGAGGAGCTA
Hairpin Sequence:
5'-CCGG-GCTCGAATATAAGAGGAGCTA-CTCGAG-TAGCTCCTCTTATATTCGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGAATATAAGAGGAGCTACTCGAGTAGCTCCTCTTATATTCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGAATATAAGAGGAGCTACTCGAGTAGCTCCTCTTATATTCGAGC-3'

Other clones with same target sequence:

(none)