Construct: shRNA TRCN0000112357

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011857.2-774s1c1
DNA Barcode:
GCAGTAATGTACCACTGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tenm3 (23965)
Gene Description:
teneurin transmembrane protein 3
Transcript:
RefSeq NM_011857.2 (NON-CURRENT)
Match location:
Position 774 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112357)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTAATGTACCACTGGAAA
Hairpin Sequence:
5'-CCGG-GCAGTAATGTACCACTGGAAA-CTCGAG-TTTCCAGTGGTACATTACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTAATGTACCACTGGAAACTCGAGTTTCCAGTGGTACATTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTAATGTACCACTGGAAACTCGAGTTTCCAGTGGTACATTACTGC-3'

Other clones with same target sequence:

(none)