Construct: shRNA TRCN0000112447

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146197.1-1179s1c1
DNA Barcode:
CCAGACAGAAACGGGACTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acsm2 (233799)
Gene Description:
acyl-CoA synthetase medium-chain family member 2
Transcript:
RefSeq NM_146197.1 (NON-CURRENT)
Match location:
Position 1179 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112447)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGACAGAAACGGGACTTAT
Hairpin Sequence:
5'-CCGG-CCAGACAGAAACGGGACTTAT-CTCGAG-ATAAGTCCCGTTTCTGTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGACAGAAACGGGACTTATCTCGAGATAAGTCCCGTTTCTGTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGACAGAAACGGGACTTATCTCGAGATAAGTCCCGTTTCTGTCTGG-3'

Other clones with same target sequence:

(none)