Construct: shRNA TRCN0000112455

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026010.1-1160s1c1
DNA Barcode:
CCTGGGTCTTTCTTCAGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lipt2 (67164)
Gene Description:
lipoyl(octanoyl) transferase 2 (putative)
Transcript:
RefSeq NM_026010.1 (NON-CURRENT)
Match location:
Position 1160 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112455)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGGTCTTTCTTCAGTAAA
Hairpin Sequence:
5'-CCGG-CCTGGGTCTTTCTTCAGTAAA-CTCGAG-TTTACTGAAGAAAGACCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGGTCTTTCTTCAGTAAACTCGAGTTTACTGAAGAAAGACCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGGTCTTTCTTCAGTAAACTCGAGTTTACTGAAGAAAGACCCAGG-3'

Other clones with same target sequence:

(none)