Construct: shRNA TRCN0000112456

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026010.1-632s1c1
DNA Barcode:
GCCATCATTCCTTGTGGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lipt2 (67164)
Gene Description:
lipoyl(octanoyl) transferase 2 (putative)
Transcript:
RefSeq NM_026010.1 (NON-CURRENT)
Match location:
Position 632 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112456)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCATTCCTTGTGGCATT
Hairpin Sequence:
5'-CCGG-GCCATCATTCCTTGTGGCATT-CTCGAG-AATGCCACAAGGAATGATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCATTCCTTGTGGCATTCTCGAGAATGCCACAAGGAATGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCATTCCTTGTGGCATTCTCGAGAATGCCACAAGGAATGATGGC-3'

Other clones with same target sequence:

(none)