Construct: shRNA TRCN0000112573

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198193.1-278s1c1
DNA Barcode:
CCTTCCCTGGTGTGACGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Raet1e (379043)
Gene Description:
retinoic acid early transcript 1E
Transcript:
RefSeq NM_198193.1 (NON-CURRENT)
Match location:
Position 278 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112573)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCCTGGTGTGACGTGAA
Hairpin Sequence:
5'-CCGG-CCTTCCCTGGTGTGACGTGAA-CTCGAG-TTCACGTCACACCAGGGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCCTGGTGTGACGTGAACTCGAGTTCACGTCACACCAGGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCCTGGTGTGACGTGAACTCGAGTTCACGTCACACCAGGGAAGG-3'

Other clones with same target sequence:

(none)