Construct: shRNA TRCN0000112602

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198610.1-665s1c1
DNA Barcode:
GCACATGGAGAATTACCGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Igsf21 (230868)
Gene Description:
immunoglobulin superfamily, member 21
Transcript:
RefSeq NM_198610.1 (NON-CURRENT)
Match location:
Position 665 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112602)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACATGGAGAATTACCGCAA
Hairpin Sequence:
5'-CCGG-GCACATGGAGAATTACCGCAA-CTCGAG-TTGCGGTAATTCTCCATGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACATGGAGAATTACCGCAACTCGAGTTGCGGTAATTCTCCATGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACATGGAGAATTACCGCAACTCGAGTTGCGGTAATTCTCCATGTGC-3'

Other clones with same target sequence:

(none)