Construct: shRNA TRCN0000112740

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023066.1-3533s1c1
DNA Barcode:
GCTGAGAAAGAGCAGTAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asph (65973)
Gene Description:
aspartate-beta-hydroxylase
Transcript:
RefSeq NM_023066.1 (NON-CURRENT)
Match location:
Position 3533 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112740)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGAAAGAGCAGTAGAAA
Hairpin Sequence:
5'-CCGG-GCTGAGAAAGAGCAGTAGAAA-CTCGAG-TTTCTACTGCTCTTTCTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGAAAGAGCAGTAGAAACTCGAGTTTCTACTGCTCTTTCTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGAAAGAGCAGTAGAAACTCGAGTTTCTACTGCTCTTTCTCAGC-3'

Other clones with same target sequence:

(none)