Construct: shRNA TRCN0000112759

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173783.2-937s1c1
DNA Barcode:
GCCCAGTAGTGGCCTCCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mageb18 (215641)
Gene Description:
melanoma antigen family B, 18
Transcript:
RefSeq NM_173783.2 (NON-CURRENT)
Match location:
Position 937 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112759)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGTAGTGGCCTCCTAAT
Hairpin Sequence:
5'-CCGG-GCCCAGTAGTGGCCTCCTAAT-CTCGAG-ATTAGGAGGCCACTACTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGTAGTGGCCTCCTAATCTCGAGATTAGGAGGCCACTACTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGTAGTGGCCTCCTAATCTCGAGATTAGGAGGCCACTACTGGGC-3'

Other clones with same target sequence:

(none)