Construct: shRNA TRCN0000112813

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011269.1-1227s1c1
DNA Barcode:
GCCACCTGATGAATACTGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rhag (19743)
Gene Description:
Rhesus blood group-associated A glycoprotein
Transcript:
RefSeq NM_011269.1 (NON-CURRENT)
Match location:
Position 1227 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112813)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCTGATGAATACTGCTA
Hairpin Sequence:
5'-CCGG-GCCACCTGATGAATACTGCTA-CTCGAG-TAGCAGTATTCATCAGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCTGATGAATACTGCTACTCGAGTAGCAGTATTCATCAGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCTGATGAATACTGCTACTCGAGTAGCAGTATTCATCAGGTGGC-3'

Other clones with same target sequence:

(none)