Construct: shRNA TRCN0000112815

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011270.2-1315s1c1
DNA Barcode:
GCCCTTTCTTTGTGTCCCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rhd (19746)
Gene Description:
Rh blood group, D antigen
Transcript:
RefSeq NM_011270.2 (NON-CURRENT)
Match location:
Position 1315 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112815)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTTCTTTGTGTCCCTTT
Hairpin Sequence:
5'-CCGG-GCCCTTTCTTTGTGTCCCTTT-CTCGAG-AAAGGGACACAAAGAAAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTTCTTTGTGTCCCTTTCTCGAGAAAGGGACACAAAGAAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTTCTTTGTGTCCCTTTCTCGAGAAAGGGACACAAAGAAAGGGC-3'

Other clones with same target sequence:

(none)