Construct: shRNA TRCN0000112866

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023544.3-130s1c1
DNA Barcode:
CCCAATAGCATTGAATCTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rsc1a1 (69994)
Gene Description:
regulatory solute carrier protein, family 1, member 1
Transcript:
RefSeq NM_023544.3 (NON-CURRENT)
Match location:
Position 130 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112866)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATAGCATTGAATCTCTA
Hairpin Sequence:
5'-CCGG-CCCAATAGCATTGAATCTCTA-CTCGAG-TAGAGATTCAATGCTATTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATAGCATTGAATCTCTACTCGAGTAGAGATTCAATGCTATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATAGCATTGAATCTCTACTCGAGTAGAGATTCAATGCTATTGGG-3'

Other clones with same target sequence:

(none)