Construct: shRNA TRCN0000112880

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172846.1-1750s1c1
DNA Barcode:
GATTGTAGAAACCCTGAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnah14 (240960)
Gene Description:
dynein, axonemal, heavy chain 14
Transcript:
RefSeq NM_172846.1 (NON-CURRENT)
Match location:
Position 1750 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112880)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTGTAGAAACCCTGAGAAA
Hairpin Sequence:
5'-CCGG-GATTGTAGAAACCCTGAGAAA-CTCGAG-TTTCTCAGGGTTTCTACAATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTGTAGAAACCCTGAGAAACTCGAGTTTCTCAGGGTTTCTACAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTGTAGAAACCCTGAGAAACTCGAGTTTCTCAGGGTTTCTACAATC-3'

Other clones with same target sequence:

(none)