Construct: shRNA TRCN0000112921

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023063.1-1260s1c1
DNA Barcode:
GCCTCACTTCAATCAACTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lima1 (65970)
Gene Description:
LIM domain and actin binding 1
Transcript:
RefSeq NM_023063.1 (NON-CURRENT)
Match location:
Position 1260 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000112921)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCACTTCAATCAACTCTT
Hairpin Sequence:
5'-CCGG-GCCTCACTTCAATCAACTCTT-CTCGAG-AAGAGTTGATTGAAGTGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCACTTCAATCAACTCTTCTCGAGAAGAGTTGATTGAAGTGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCACTTCAATCAACTCTTCTCGAGAAGAGTTGATTGAAGTGAGGC-3'

Other clones with same target sequence:

(none)