Construct: shRNA TRCN0000113043

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007839.1-2264s1c1
DNA Barcode:
GCTGACGACAAAGAATTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dhx15 (13204)
Gene Description:
DEAH (Asp-Glu-Ala-His) box polypeptide 15
Transcript:
RefSeq NM_007839.1 (NON-CURRENT)
Match location:
Position 2264 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113043)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACGACAAAGAATTATAT
Hairpin Sequence:
5'-CCGG-GCTGACGACAAAGAATTATAT-CTCGAG-ATATAATTCTTTGTCGTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACGACAAAGAATTATATCTCGAGATATAATTCTTTGTCGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACGACAAAGAATTATATCTCGAGATATAATTCTTTGTCGTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000298333