Construct: shRNA TRCN0000113072

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052976.2-1478s1c1
DNA Barcode:
GCGAAATACTATTGTCGGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ophn1 (94190)
Gene Description:
oligophrenin 1
Transcript:
RefSeq NM_052976.2 (NON-CURRENT)
Match location:
Position 1478 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113072)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAAATACTATTGTCGGTAT
Hairpin Sequence:
5'-CCGG-GCGAAATACTATTGTCGGTAT-CTCGAG-ATACCGACAATAGTATTTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAAATACTATTGTCGGTATCTCGAGATACCGACAATAGTATTTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAAATACTATTGTCGGTATCTCGAGATACCGACAATAGTATTTCGC-3'

Other clones with same target sequence:

(none)