Construct: shRNA TRCN0000113122

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021710.1-527s1c1
DNA Barcode:
CGGCAATATGCAGCTCTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap4s1 (11782)
Gene Description:
adaptor-related protein complex AP-4, sigma 1
Transcript:
RefSeq NM_021710.1 (NON-CURRENT)
Match location:
Position 527 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113122)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAATATGCAGCTCTCTTT
Hairpin Sequence:
5'-CCGG-CGGCAATATGCAGCTCTCTTT-CTCGAG-AAAGAGAGCTGCATATTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAATATGCAGCTCTCTTTCTCGAGAAAGAGAGCTGCATATTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAATATGCAGCTCTCTTTCTCGAGAAAGAGAGCTGCATATTGCCG-3'

Other clones with same target sequence:

(none)