Construct: shRNA TRCN0000113148

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009938.2-3277s1c1
DNA Barcode:
GCCGTGAATACATTGTGGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Copa (12847)
Gene Description:
coatomer protein complex subunit alpha
Transcript:
RefSeq NM_009938.2 (NON-CURRENT)
Match location:
Position 3277 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113148)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTGAATACATTGTGGGTT
Hairpin Sequence:
5'-CCGG-GCCGTGAATACATTGTGGGTT-CTCGAG-AACCCACAATGTATTCACGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTGAATACATTGTGGGTTCTCGAGAACCCACAATGTATTCACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTGAATACATTGTGGGTTCTCGAGAACCCACAATGTATTCACGGC-3'

Other clones with same target sequence:

(none)