Construct: shRNA TRCN0000113158

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013669.1-2200s1c1
DNA Barcode:
GCTCAGAATAACCTGCTGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snap91 (20616)
Gene Description:
synaptosomal-associated protein 91
Transcript:
RefSeq NM_013669.1 (NON-CURRENT)
Match location:
Position 2200 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113158)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAGAATAACCTGCTGCAA
Hairpin Sequence:
5'-CCGG-GCTCAGAATAACCTGCTGCAA-CTCGAG-TTGCAGCAGGTTATTCTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAGAATAACCTGCTGCAACTCGAGTTGCAGCAGGTTATTCTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAGAATAACCTGCTGCAACTCGAGTTGCAGCAGGTTATTCTGAGC-3'

Other clones with same target sequence:

(none)