Construct: shRNA TRCN0000113188

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146194.2-633s1c1
DNA Barcode:
GCTTCAAGAAACACATTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Picalm (233489)
Gene Description:
phosphatidylinositol binding clathrin assembly protein
Transcript:
RefSeq NM_146194.2 (NON-CURRENT)
Match location:
Position 633 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113188)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCAAGAAACACATTGTTT
Hairpin Sequence:
5'-CCGG-GCTTCAAGAAACACATTGTTT-CTCGAG-AAACAATGTGTTTCTTGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCAAGAAACACATTGTTTCTCGAGAAACAATGTGTTTCTTGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCAAGAAACACATTGTTTCTCGAGAAACAATGTGTTTCTTGAAGC-3'

Other clones with same target sequence:

  1. TRCN0000312300