Construct: shRNA TRCN0000113225

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026319.1-1093s1c1
DNA Barcode:
GCCGACTGAGTCTACAAGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ift74 (67694)
Gene Description:
intraflagellar transport 74
Transcript:
RefSeq NM_026319.1 (NON-CURRENT)
Match location:
Position 1093 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113225)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGACTGAGTCTACAAGCAT
Hairpin Sequence:
5'-CCGG-GCCGACTGAGTCTACAAGCAT-CTCGAG-ATGCTTGTAGACTCAGTCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGACTGAGTCTACAAGCATCTCGAGATGCTTGTAGACTCAGTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGACTGAGTCTACAAGCATCTCGAGATGCTTGTAGACTCAGTCGGC-3'

Other clones with same target sequence:

  1. TRCN0000288196