Construct: shRNA TRCN0000113287

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174875.2-112s1c1
DNA Barcode:
CGGATACAGATGAGCTGGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atg4a (103003)
Gene Description:
autophagy-related 4A (yeast)
Transcript:
RefSeq NM_174875.2 (NON-CURRENT)
Match location:
Position 112 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113287)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATACAGATGAGCTGGTAT
Hairpin Sequence:
5'-CCGG-CGGATACAGATGAGCTGGTAT-CTCGAG-ATACCAGCTCATCTGTATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATACAGATGAGCTGGTATCTCGAGATACCAGCTCATCTGTATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATACAGATGAGCTGGTATCTCGAGATACCAGCTCATCTGTATCCG-3'

Other clones with same target sequence:

(none)