Construct: shRNA TRCN0000113334

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175185.2-992s1c1
DNA Barcode:
AGTTCCTCTTCGCACAGTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hsdl1 (72552)
Gene Description:
hydroxysteroid dehydrogenase like 1
Transcript:
RefSeq NM_175185.2 (NON-CURRENT)
Match location:
Position 992 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113334)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTCCTCTTCGCACAGTATA
Hairpin Sequence:
5'-CCGG-AGTTCCTCTTCGCACAGTATA-CTCGAG-TATACTGTGCGAAGAGGAACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTCCTCTTCGCACAGTATACTCGAGTATACTGTGCGAAGAGGAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTCCTCTTCGCACAGTATACTCGAGTATACTGTGCGAAGAGGAACT-3'

Other clones with same target sequence:

(none)