Construct: shRNA TRCN0000113340

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021449.1-1422s1c1
DNA Barcode:
CCTACCAAGTTCAAGAGCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crbn (58799)
Gene Description:
cereblon
Transcript:
RefSeq NM_021449.1 (NON-CURRENT)
Match location:
Position 1422 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113340)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACCAAGTTCAAGAGCATA
Hairpin Sequence:
5'-CCGG-CCTACCAAGTTCAAGAGCATA-CTCGAG-TATGCTCTTGAACTTGGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACCAAGTTCAAGAGCATACTCGAGTATGCTCTTGAACTTGGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACCAAGTTCAAGAGCATACTCGAGTATGCTCTTGAACTTGGTAGG-3'

Other clones with same target sequence:

  1. TRCN0000316158