Construct: shRNA TRCN0000113372

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172751.1-2470s1c1
DNA Barcode:
CCTGAACCTTACCTGAGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgef10 (234094)
Gene Description:
Rho guanine nucleotide exchange factor (GEF) 10
Transcript:
RefSeq NM_172751.1 (NON-CURRENT)
Match location:
Position 2470 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113372)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAACCTTACCTGAGCAAT
Hairpin Sequence:
5'-CCGG-CCTGAACCTTACCTGAGCAAT-CTCGAG-ATTGCTCAGGTAAGGTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAACCTTACCTGAGCAATCTCGAGATTGCTCAGGTAAGGTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAACCTTACCTGAGCAATCTCGAGATTGCTCAGGTAAGGTTCAGG-3'

Other clones with same target sequence:

(none)