Construct: shRNA TRCN0000113427

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_145984.2-1206s1c1
DNA Barcode:
CCGGGAATCATTGGTCATGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abca16 (233810)
Gene Description:
ATP-binding cassette, sub-family A (ABC1), member 16
Transcript:
RefSeq XM_145984.2 (NON-CURRENT)
Match location:
Position 1206 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113427)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGGAATCATTGGTCATGTA
Hairpin Sequence:
5'-CCGG-CCGGGAATCATTGGTCATGTA-CTCGAG-TACATGACCAATGATTCCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGGAATCATTGGTCATGTACTCGAGTACATGACCAATGATTCCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGGAATCATTGGTCATGTACTCGAGTACATGACCAATGATTCCCGG-3'

Other clones with same target sequence:

(none)