Construct: shRNA TRCN0000113440

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_147220.1-5209s1c1
DNA Barcode:
CGGTGTGCAAACAGCTTAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abca9 (217262)
Gene Description:
ATP-binding cassette, sub-family A (ABC1), member 9
Transcript:
RefSeq NM_147220.1 (NON-CURRENT)
Match location:
Position 5209 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113440)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGTGCAAACAGCTTAGAA
Hairpin Sequence:
5'-CCGG-CGGTGTGCAAACAGCTTAGAA-CTCGAG-TTCTAAGCTGTTTGCACACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGTGCAAACAGCTTAGAACTCGAGTTCTAAGCTGTTTGCACACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGTGCAAACAGCTTAGAACTCGAGTTCTAAGCTGTTTGCACACCG-3'

Other clones with same target sequence:

(none)