Construct: shRNA TRCN0000113449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019552.2-1512s1c1
DNA Barcode:
GTACGCTTTCTGGGTTGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abcb10 (56199)
Gene Description:
ATP-binding cassette, sub-family B (MDR/TAP), member 10
Transcript:
RefSeq NM_019552.2 (CURRENT)
Match location:
Position 1512 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACGCTTTCTGGGTTGGATT
Hairpin Sequence:
5'-CCGG-GTACGCTTTCTGGGTTGGATT-CTCGAG-AATCCAACCCAGAAAGCGTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACGCTTTCTGGGTTGGATTCTCGAGAATCCAACCCAGAAAGCGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACGCTTTCTGGGTTGGATTCTCGAGAATCCAACCCAGAAAGCGTAC-3'

Other clones with same target sequence:

(none)