Construct: shRNA TRCN0000113457

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175210.2-631s1c1
DNA Barcode:
CGCTATTTAAAGAAAGCGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abca12 (74591)
Gene Description:
ATP-binding cassette, sub-family A (ABC1), member 12
Transcript:
RefSeq NM_175210.2 (NON-CURRENT)
Match location:
Position 631 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113457)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTATTTAAAGAAAGCGAAA
Hairpin Sequence:
5'-CCGG-CGCTATTTAAAGAAAGCGAAA-CTCGAG-TTTCGCTTTCTTTAAATAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTATTTAAAGAAAGCGAAACTCGAGTTTCGCTTTCTTTAAATAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTATTTAAAGAAAGCGAAACTCGAGTTTCGCTTTCTTTAAATAGCG-3'

Other clones with same target sequence:

(none)