Construct: shRNA TRCN0000113517

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011639.2-1080s1c1
DNA Barcode:
TGTCTTCCATATTGGTTGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trip6 (22051)
Gene Description:
thyroid hormone receptor interactor 6
Transcript:
RefSeq NM_011639.2 (NON-CURRENT)
Match location:
Position 1080 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113517)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCTTCCATATTGGTTGCTT
Hairpin Sequence:
5'-CCGG-TGTCTTCCATATTGGTTGCTT-CTCGAG-AAGCAACCAATATGGAAGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCTTCCATATTGGTTGCTTCTCGAGAAGCAACCAATATGGAAGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCTTCCATATTGGTTGCTTCTCGAGAAGCAACCAATATGGAAGACA-3'

Other clones with same target sequence:

(none)