Construct: shRNA TRCN0000113604

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020486.1-1554s1c1
DNA Barcode:
GTCCTGTGAAGCGTCTAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bcam (57278)
Gene Description:
basal cell adhesion molecule
Transcript:
RefSeq NM_020486.1 (NON-CURRENT)
Match location:
Position 1554 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113604)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCTGTGAAGCGTCTAACAT
Hairpin Sequence:
5'-CCGG-GTCCTGTGAAGCGTCTAACAT-CTCGAG-ATGTTAGACGCTTCACAGGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCTGTGAAGCGTCTAACATCTCGAGATGTTAGACGCTTCACAGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCTGTGAAGCGTCTAACATCTCGAGATGTTAGACGCTTCACAGGAC-3'

Other clones with same target sequence:

  1. TRCN0000325768