Construct: shRNA TRCN0000113606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177350.2-1514s1c1
DNA Barcode:
CCTTTGATTTGTTAGGAGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gldn (235379)
Gene Description:
gliomedin
Transcript:
RefSeq NM_177350.2 (NON-CURRENT)
Match location:
Position 1514 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGATTTGTTAGGAGGAA
Hairpin Sequence:
5'-CCGG-CCTTTGATTTGTTAGGAGGAA-CTCGAG-TTCCTCCTAACAAATCAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGATTTGTTAGGAGGAACTCGAGTTCCTCCTAACAAATCAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGATTTGTTAGGAGGAACTCGAGTTCCTCCTAACAAATCAAAGG-3'

Other clones with same target sequence:

(none)