Construct: shRNA TRCN0000113611

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177059.3-1291s1c1
DNA Barcode:
GCCCAGAATCATTTGGCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fstl4 (320027)
Gene Description:
follistatin-like 4
Transcript:
RefSeq NM_177059.3 (CURRENT)
Match location:
Position 1291 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113611)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGAATCATTTGGCTGAA
Hairpin Sequence:
5'-CCGG-GCCCAGAATCATTTGGCTGAA-CTCGAG-TTCAGCCAAATGATTCTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGAATCATTTGGCTGAACTCGAGTTCAGCCAAATGATTCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGAATCATTTGGCTGAACTCGAGTTCAGCCAAATGATTCTGGGC-3'

Other clones with same target sequence:

(none)