Construct: shRNA TRCN0000113637

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177906.3-378s1c1
DNA Barcode:
GCCTACCCAATACAGTATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Opcml (330908)
Gene Description:
opioid binding protein/cell adhesion molecule-like
Transcript:
RefSeq NM_177906.3 (NON-CURRENT)
Match location:
Position 378 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113637)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTACCCAATACAGTATCAT
Hairpin Sequence:
5'-CCGG-GCCTACCCAATACAGTATCAT-CTCGAG-ATGATACTGTATTGGGTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTACCCAATACAGTATCATCTCGAGATGATACTGTATTGGGTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTACCCAATACAGTATCATCTCGAGATGATACTGTATTGGGTAGGC-3'

Other clones with same target sequence:

(none)