Construct: shRNA TRCN0000113781

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014845.4-2938s1c1
DNA Barcode:
GAACCTGTCTTGTCTCATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FIG4 (9896)
Gene Description:
FIG4 phosphoinositide 5-phosphatase
Transcript:
RefSeq NM_014845.4 (NON-CURRENT)
Match location:
Position 2938 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113781)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCTGTCTTGTCTCATCTT
Hairpin Sequence:
5'-CCGG-GAACCTGTCTTGTCTCATCTT-CTCGAG-AAGATGAGACAAGACAGGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCTGTCTTGTCTCATCTTCTCGAGAAGATGAGACAAGACAGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCTGTCTTGTCTCATCTTCTCGAGAAGATGAGACAAGACAGGTTC-3'

Other clones with same target sequence:

  1. TRCN0000299149