Construct: shRNA TRCN0000113833

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020866.1-1850s1c1
DNA Barcode:
CCACAGATATTGGCTGACCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KLHL1 (57626)
Gene Description:
kelch like family member 1
Transcript:
RefSeq NM_020866.1 (NON-CURRENT)
Match location:
Position 1850 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113833)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGATATTGGCTGACCTA
Hairpin Sequence:
5'-CCGG-CCACAGATATTGGCTGACCTA-CTCGAG-TAGGTCAGCCAATATCTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGATATTGGCTGACCTACTCGAGTAGGTCAGCCAATATCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGATATTGGCTGACCTACTCGAGTAGGTCAGCCAATATCTGTGG-3'

Other clones with same target sequence:

(none)