Construct: shRNA TRCN0000113904

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020242.1-2124s1c1
DNA Barcode:
GCTCTCTATACACTCAGAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIF15 (56992)
Gene Description:
kinesin family member 15
Transcript:
RefSeq NM_020242.1 (NON-CURRENT)
Match location:
Position 2124 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113904)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCTATACACTCAGAATT
Hairpin Sequence:
5'-CCGG-GCTCTCTATACACTCAGAATT-CTCGAG-AATTCTGAGTGTATAGAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCTATACACTCAGAATTCTCGAGAATTCTGAGTGTATAGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCTATACACTCAGAATTCTCGAGAATTCTGAGTGTATAGAGAGC-3'

Other clones with same target sequence:

(none)