Construct: shRNA TRCN0000113918

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006088.5-1078s1c1
DNA Barcode:
GTGGATGAGCAAATGCTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TUBB4B (10383)
Gene Description:
tubulin beta 4B class IVb
Transcript:
RefSeq NM_006088.5 (NON-CURRENT)
Match location:
Position 1078 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113918)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGATGAGCAAATGCTTAAT
Hairpin Sequence:
5'-CCGG-GTGGATGAGCAAATGCTTAAT-CTCGAG-ATTAAGCATTTGCTCATCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGATGAGCAAATGCTTAATCTCGAGATTAAGCATTTGCTCATCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGATGAGCAAATGCTTAATCTCGAGATTAAGCATTTGCTCATCCAC-3'

Other clones with same target sequence:

  1. TRCN0000290911