Construct: shRNA TRCN0000113933

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015282.1-3556s1c1
DNA Barcode:
CCTGAATTTACCATGTTACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLASP1 (23332)
Gene Description:
cytoplasmic linker associated protein 1
Transcript:
RefSeq NM_015282.1 (NON-CURRENT)
Match location:
Position 3556 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113933)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAATTTACCATGTTACTT
Hairpin Sequence:
5'-CCGG-CCTGAATTTACCATGTTACTT-CTCGAG-AAGTAACATGGTAAATTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAATTTACCATGTTACTTCTCGAGAAGTAACATGGTAAATTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAATTTACCATGTTACTTCTCGAGAAGTAACATGGTAAATTCAGG-3'

Other clones with same target sequence:

(none)