Construct: shRNA TRCN0000113951

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018807.4-4129s1c1
DNA Barcode:
GCTGGTTATCTGCCTTCAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plagl2 (54711)
Gene Description:
pleiomorphic adenoma gene-like 2
Transcript:
RefSeq NM_018807.4 (NON-CURRENT)
Match location:
Position 4129 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000113951)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGTTATCTGCCTTCAATT
Hairpin Sequence:
5'-CCGG-GCTGGTTATCTGCCTTCAATT-CTCGAG-AATTGAAGGCAGATAACCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGTTATCTGCCTTCAATTCTCGAGAATTGAAGGCAGATAACCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGTTATCTGCCTTCAATTCTCGAGAATTGAAGGCAGATAACCAGC-3'

Other clones with same target sequence:

(none)